Synthesis of ZnO/Ag Nanocomposites with Variatons of Zinc Nitrate Hexahydrate as an Antibacterial Agent against Escherichia coli

Zeiiffa Alifia Maulita; Posman Manurung; Suprihatin; Dwi Asmi

doi:10.23960/jemit.368

Authors

Zeiiffa Alifia Maulita Department of Physics, Faculty of Mathematics and Natural Sciences, University of Lampung, Bandar Lampung, Indonesia, 35141
Posman Manurung Department of Physics, Faculty of Mathematics and Natural Sciences, University of Lampung, Bandar Lampung, Indonesia, 35141
Suprihatin Department of Physics, Faculty of Mathematics and Natural Sciences, University of Lampung, Bandar Lampung, Indonesia, 35141
Dwi Asmi Department of Physics, Faculty of Mathematics and Natural Sciences, University of Lampung, Bandar Lampung, Indonesia, 35141

DOI:

https://doi.org/10.23960/jemit.368

Keywords:

antibacterial, e.coli, nanocomposites, silver, zinc oxide

Abstract

The synthesis of ZnO/Ag nanocomposites was carried out using different concentrations of Zn(NO₃)₂.6H₂O precursors (0.04 M, 0.06 M, 0.08 M, and 0.1 M) by wet chemical precipitation. This study aimed to characterize the structure, morphology, and antibacterial activity of ZnO/Ag nanocomposites against Escherichia coli (E.coli). The characterization techniques used were X-Ray Fluorescence (XRF), X-Ray Diffraction (XRD), and Scanning Electron Microscopy with Energy Dispersive X-Ray (SEM-EDX). XRF analysis confirmed the presence of Zn as the main element and Ag as a dopant. XRD results showed that the ZnO/Ag nanocomposites exhibited a hexagonal wurtzite structure for ZnO and a Face-Centered Cubic (FCC) structure for Ag. SEM-EDX analysis revealed homogeneously distributed particles with nano-scale morphology. Antibacterial activity was tested using the disk diffusion method. The results indicated that the inhibition zone increased with higher Zn(NO₃)₂.6H₂O concentration, proving that ZnO/Ag nanocomposites exhibit significant antibacterial properties against E.coli.

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